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Here we describe a novel and more cost-effective method for siRNA preparation and its application in inhibition of the replication of hepatitis B virus in HepG2 cells. In this paper we describe a novel cost-effective siRNA preparation method. We showed that dsRNAs can be easily prepared by fermentation in E.coli and purified by affinity chromatography. In conclusion the novel siRNA preparation method makes it possible to produce siRNAs on a large.

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Keywords relevant to Sirna Preparation Form

  • siRNA
  • mMol
  • 2003
  • transfection
  • dsrna
  • HBV
  • dsRNAs
  • siHBVP
  • HepG2
  • rnas
  • SMMC7721
  • SEAP
  • transfected
  • Xho
  • CFDP
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