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PowerUp SYBR Green Master Mix USER GUIDE Universal 2X master mix for realtime PCR workflows Catalog Numbers A25741, A25742, A25743, A25776, A25777, A25778, A25779, A25780, A25918 Publication Number.

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SYBR Green master mixes are a fast and convenient means of carrying out quantitative PCR (qPCR) experiments. SYBR Green is a cyanine fluorophore that intercalates with and stains double-stranded DNA. Measurement of SYBR fluorescence intensity after each amplification cycle allows quantification of the amount of DNA.

This method obviates the need for qPCR probes, making its use less expensive. Its major disadvantage is that specificity is determined entirely by the primers and thus the risk of amplifying nonspecific PCR products has to be considered during optimization. Figure 1. Principle of detection using Sybr Green.

It contains the following components: SYBR Green 1 dye, AmpliTaq Gold DNA polymerase, dNTPs (with dUTP), Passive Reference 1 and optimized buffer components; all the necessary components to perform real-time PCR in a convenient premixed 2X solution.

PCR Master Mix is a premixed, ready-to-use solution containing Taq DNA polymerase, dNTPs, MgCl2 and reaction buffers at optimal concentrations for efficient amplification of DNA templates by PCR.

Components. A PCR master mix consists of a thermostable DNA polymerase, optimized reaction buffers, dNTPs, and MgCl2 (or often MgSO4).

SYBR® Green Fluorescent DNA Stain is supplied as 100 μM concentration. Vortex SYBR® Green Fluorescent DNA Stain thoroughly prior to its use. An SYBR® Green concentration of 0.5-1.0 μM in the final assay is recommended.

SYBR® Green Master Mix Advantages SYBR® Green dye is a fluorescent double-stranded DNA (dsDNA)- binding dye that is used to track the progress of DNA amplification in real-time PCR experiments.

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Keywords relevant to Powerup Sybr Green Master Mix

  • Powerup
  • cDNA
  • RNA
  • Polymerase
  • UdG
  • ntc
  • nm
  • amplicon
  • ThermoFisher
  • quantitation
  • 95C
  • Absorbance
  • mls
  • DRN
  • optimizing
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© Copyright 1997-2025
airSlate Legal Forms, Inc.
3720 Flowood Dr, Flowood, Mississippi 39232
Form Packages
Adoption
Bankruptcy
Contractors
Divorce
Home Sales
Employment
Identity Theft
Incorporation
Landlord Tenant
Living Trust
Name Change
Personal Planning
Small Business
Wills & Estates
Packages A-Z
Form Categories
Affidavits
Bankruptcy
Bill of Sale
Corporate - LLC
Divorce
Employment
Identity Theft
Internet Technology
Landlord Tenant
Living Wills
Name Change
Power of Attorney
Real Estate
Small Estates
Wills
All Forms
Forms A-Z
Form Library
Customer Service
Terms of Service
DMCA Policy
About Us
Blog
Affiliates
Contact Us
Privacy Notice
Delete My Account
Site Map
All Forms
Search all Forms
Industries
Forms in Spanish
Localized Forms
Legal Guides
Real Estate Handbook
All Guides
Prepared for You
Notarize
Incorporation services
Our Customers
For Consumers
For Small Business
For Attorneys
Our Sites
US Legal Forms
USLegal
FormsPass
pdfFiller
signNow
airSlate workflows
DocHub
Instapage
Social Media
Call us now toll free:
1-877-389-0141
As seen in:
  • USA Today logo picture
  • CBC News logo picture
  • LA Times logo picture
  • The Washington Post logo picture
  • AP logo picture
  • Forbes logo picture
© Copyright 1997-2025
airSlate Legal Forms, Inc.
3720 Flowood Dr, Flowood, Mississippi 39232