Title VII prohibits employment discrimination based on race, color, religion, sex and national origin. Title VII of the Civil Rights Act of 1964 outlaws discrimination against a job seeker or employee on the basis of race, color, religion, sex.Employers who have engaged in unlawful discrimination may be subject to monetary fines from the EEOC. A) Discriminatory practices prohibited; employees or applicants for employment subject to coverage. The act made it illegal to discriminate in the workplace based on a person's race, color, religion, sex and national origin. Discrimination under Title VII of the Civil Rights Act of 1964, as amended.