Title VII of the Civil Rights Act of 1964, as amended applies to employers with fifteen (15) or more employees. Title VII of the Civil Rights Act of 1964 prohibits employment discrimination based on race, color, religion, sex and national origin.These cases can offer helpful guidance for employers. Employment Discrimination—Disparate Treatment. Virginia's Human Rights Act and several federal laws, including the Civil Rights Act of 1964, prohibit certain types of employment-based discrimination. Title VII of the Civil Rights Act of 1964. Virginia's Human Rights Act and several federal laws, including the Civil Rights Act of 1964, prohibit certain types of employment-based discrimination. Title VII of the Civil Rights Act of 1964.