Title VII prohibits employment discrimination based on race, color, religion, sex and national origin. TITLE VII--EQUAL EMPLOYMENT OPPORTUNITY. DEFINITIONS. SEC. 701.For the purposes of this title--. A) Discriminatory practices prohibited; employees or applicants for employment subject to coverage. The Civil Rights Act of 1964 hastened the end of legal Jim Crow. Title VII prohibits racial discrimination, including bias on the basis of ancestry or ethnic characteristics such as skin color and facial features. Title VII of the Civil Rights Act of 1964 is a federal law that protects employees against discrimination based on certain specified characteristics. Title VII of the Civil Rights Act of 1964 prohibits employment discrimination based on race, color, religion, sex, and national origin. Title VII has expanded to protect employees from all forms of discrimination in the workplace. Discrimination laws, including Title VII of the 1964 federal Civil Rights Act (codified as 42 U.S.C.. § 2000e et seq.).