Title VII prohibits employment discrimination based on race, color, religion, sex and national origin. Title VII of the Civil Rights Act of 1964 prohibits employment discrimination based on race, color, religion, sex, and national origin.A) Discriminatory practices prohibited; employees or applicants for employment subject to coverage. Doe asserts federal statutory claims under Title VII of the Civil Rights Act of. 1964 ("Title VII"), 42 U.S.C. § 2000e et seq. It banned discriminatory practices in employment and ended segregation in public places such as swimming pools, libraries, and public schools. The Civil Rights Act of 1964 prohibited discrimination based on race, color, religion, sex, and national origin. Later laws added more protections.