Professional written notice of at least two weeks is expected. The final day of employment will be the last actual day worked; it may not be a day of time off.If they decide to terminate your employment immediately, that's up to them. But don't use your vacation as part of your two week notice period. There's also the option to turn your "2 weeks notice" into a "3 weeks notice": state your intention to leave 2 weeks before your PTO starts. Can you use vacation time for 2 weeks' notice? Managers may be able to deny PTO requests from employees who resign with a 2-week notice letter. Learn how to write a professional two weeks notice letter with our easytouse template. The best situation is to wait for the boss to get back and provide the letter to them, and letting the new company know about the delay.