In this step, the PCR mixture is incubated at the extension temperature (generally 72°C) for a final 5–15 minute period. Therefore, using a lower extension temperature of 68°C instead of 72°C dramatically improves yield of longer amplification products.Use a denaturing temperature of 92°C, an extension temperature of 68°C, and an extension time of 2.0 minutes per kilobase. Open the application forms in the table below to view the submittal requirements. When temperature is set below 68°C, a longer extension time will be required. The advantage of using the. Accelerate your discoveries with our dPCR, qPCR, and PCR reagents including master mixes, predesigned assays, and custom primers and probes.