Utah Aeseptic Techniques
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Overviewdecontaminate your lab bench.safely organize your workspace.properly adjust your Bunsen burner.properly use an incinerator.sterilize your inoculating tools.aseptically transfer organisms from broth/plate cultures using BSL 2 procedures.handle biohazard spills and dispose of biohazard materials.
Aseptic techniques include:Wiping bench with disinfectant/alcohol. Not growing microorganisms at body temperature. Using sterile loops when transferring cultures . Flaming culture bottle necks to prevent contamination. Sterilising (using an autoclave ) or disposing of all used equipment.
Aseptic technique is a method that involves target-specific practices and procedures under suitably controlled conditions to reduce the contamination from microbes. It is a compulsory laboratory skill to conduct research related in the field of microbiology.
These principles include the following: (1) use only sterile items within a sterile field; (2) sterile (scrubbed) personnel are gowned and gloved; (3) sterile personnel operate within a sterile field (sterile personnel touch only sterile items or areas, unsterile personnel touch only unsterile items or areas); (4)
Aseptic technique is classified into two different categories: standard aseptic technique and surgical aseptic technique.
Procedures that involve aseptic technique include:200cInserting PICC lines.200cPerforming dialysis.200cInserting catheters.200cRunning IVs.200cInserting chest tubes.200cPerforming surgeries.200cDressing wounds.
Standard aseptic technique. The most common type of aseptic technique, this is the sterilization process used for things like dialysis or IV insertions. Doctors use handwashing, small aseptic fields, and masks and gloves to keep these small areas free from germs.
There are some general rules to follow for any aseptic technique. Close windows and doors to reduce draughts and prevent sudden movements which might disturb the air. Make transfers over a disinfected surface. Ethanol disinfection is recommended because of its rapid action.
Healthcare professionals use aseptic technique when they are:performing surgical procedures.performing biopsies.dressing surgical wounds or burns.suturing wounds.inserting a urinary catheter, wound drain, intravenous line, or chest tube.administering injections.using instruments to conduct a vaginal examination.More items...?