Alter Equation Resolution For Free

Peak resolution is a key parameter in high-performance liquid chromatography (HPLC) data analysis, as it measures the degree of separation between two adjacent peaks in a chromatogram.

If better resolution is desired, reduce the velocity to no less than 25 cm/sec; however, the analysis time will be increased. If a shorter analysis time is desired, increase the velocity to 35 cm/sec and 40 cm/sec. Be aware of potential resolution losses at these higher linear velocities.

Resolution is an important HPLC performance indicator usually assessed by how quickly and how completely target components in a sample separate as they pass through a column. Resolution is measured by dividing the difference in peak retention times by the average peak width.

Generally, increasing the organic solvent content, decreasing the pH, and decreasing the flow rate improve peak resolution, but they also affect the retention time, peak shape, and sensitivity. Therefore, you need to optimize these parameters by performing a gradient elution, a buffer titration, or a flow optimization.

Equation (1) indicates that the resolution is the difference between peak retention times divided by the average peak width. In a peak with Gaussian distribution, the peak width is W = 4 ? (where ? is the standard deviation) and the peak FWHM is W0.

In chromatography, resolution is a measure of the separation of two peaks of different retention time t in a chromatogram.

The "Resolution Equation" in chromatography describes the factors which influence the separation of two analytes. A common form of this equation is given below: R s = N 2 ( k 1 + k ) ( ? ? 1 ) ( ? + 1 ) .