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Resolution. The resolution of a elution is a quantitative measure of how well two elution peaks can be differentiated in a chromatographic separation. It is defined as the difference in retention times between the two peaks, divided by the combined widths of the elution peaks.

A good selectivity for HPLC is 1.1, which allows a resolution of 1.5 to be achieved with about 10,000 theoretical places. The critical pair in a separation is defined as adjacent solutes that have the smallest 03b1 value.

In general, good chromatography has baseline separation between peaks, and peaks should be symmetric. A long tail on the end of a peak may mean that the sample is interacting with the column material, too much sample has been injected (column overload), or column performance is reduced (column aging).

K) The resolution (Rs) of a column for two analytes (A eluting before B) is given by Rs = tR(B) - tR(A)/(WA + WB)/2, where W is the baseline width of a chromatographic peak in the same units as the analyte retention time.

In chromatography, resolution is a measure of the separation of two peaks of different retention time t in a chromatogram.

Resolution. The resolution of a elution is a quantitative measure of how well two elution peaks can be differentiated in a chromatographic separation. It is defined as the difference in retention times between the two peaks, divided by the combined widths of the elution peaks. RS=2(tR)B2212(tR)AWB+WA.

In chromatography, resolution is a measure of the separation of two peaks of different retention time t in a chromatogram.