Dispose Columns Release For Free

5.6 Discard of HPLC / GC column 1 Discard the column if it is not meeting the criteria of Tailing factor, theoretical plates, Resolution, Fronting, Splitting/ damage etc. 5.6. 3 Discarded columns shall be kept separately and affixed to the label as ?Discarded column-Do not Use?.

If the column was properly flushed after use and stored in 100% organic solvent such as acetonitrile, the column will survive for many years.

Below are some of the signs to look for that your relationship and your HPLC column lifetime is coming to an end. High Backpressure not reduced by reverse flushing the column. Split Peaks. Loss of resolution. Broad peak shape. Retention time shifts.

Symptoms of deterioration are poor peak shape, split peaks, shoulders, loss of resolution, decreased retention times, and high back pressure. These symptoms indicate contaminants have accumulated on the frit or column inlet, or there are voids, channels, or a depression in the packing bed.

Laminar flow or drag caused by the tubing wall can cause band broadening outside of the column bed. Too much connection tubing or tubing with too large a diameter can significantly decrease observed resolution.

HPLC column washing method for normal phase mode. - YouTube YouTube Start of suggested clip End of suggested clip For the first washing method we recommend choosing isopropanol or ethanol these are easily mixedMoreFor the first washing method we recommend choosing isopropanol or ethanol these are easily mixed with most solvents such as hexane used in normal phase. Mode.

We recommend consulting a chemical disposal company with the MSDS for the packing material and any other chemicals that might also be in the column. It may be possible to then flush the column, properly dispose of its contents and recycle the hardware.