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Resolution is measured by dividing the difference in peak retention times by the average peak width. Resolution can also be expressed in the Resolution Equation as a combination of the factors (separation, efficiency, and retention) that affect this value.

A good selectivity for HPLC is 1.1, which allows a resolution of 1.5 to be achieved with about 10,000 theoretical places. The critical pair in a separation is defined as adjacent solutes that have the smallest ? value.

Resolution. The resolution of a elution is a quantitative measure of how well two elution peaks can be differentiated in a chromatographic separation. It is defined as the difference in retention times between the two peaks, divided by the combined widths of the elution peaks.

Poor chromatograms are those with early peaks (small tr) that are broad (large w), hence giving small N values, while excellent chromatograms are those with late-appearing peaks (large tr) that are still very narrow (small w), thereby producing a large N.

D= ?/2 NA. Where ? is the wavelength of light used to image a specimen. If using a green light of 514 nm and an oil immersion objective with an NA of 1.45, then the (theoretical) limit of resolution will be 177 nm. Abbe's diffraction formula for axial (i.e. Z) resolution is: d= 2 ?/NA2.

In many cases, the best resolution for printing is 300 PPI. At 300 pixels per inch (which roughly translates to 300 DPI, or dots per inch, on a printing press), an image will appear sharp and crisp. These are considered to be high resolution, or high-res, images.

Scientists consider a resolution of 1.0 or higher to represent an adequate separation. Measure the widths of two adjacent peaks in the chromatogram by noting where the x-axis values are at the base of each peak. The x-axis represents retention time, usually measured in seconds.