Extend Columns Resolution For Free

Chromatographic parameters The advantage of switching to longer columns is that the theoretical plates will increase and so will the resolution.

Increase in column length increases the resolution and increase in column diameter results in high bed volume and hence higher column capacity. The fractionation range and the exclusion limit can be controlled by varying pore size. The smaller the particle size of the gel, the higher the resolution achieved.

The advantage of switching to longer columns is that the theoretical plates will increase and so will the resolution. The number of theoretical plates (N) is directly proportional to the length of the column and inversely proportional to the particle diameter2.

A longer column generally improves the separation. The trade-off is that the retention time increases proportionally to the column length and a significant peak broadening will be observed as well because of increased longitudinal diffusion inside the column.

Column length and efficiency This has the effect of reducing the run time ? and so the throughput of the system is increased. If the column length is reduced, the number of theoretical plates in the column will be correspondingly reduced and therefore the resolution of the HPLC system.

Another way to improve column efficiency is to elevate column temperature. Higher column temperatures reduce mobile phase viscosity and increase solvent diffusion rate, therefore increasing column efficiency.

Factors Governing the Resolution of peaks in the Gas Chromatogram Boiling Point. Boiling point is the temperature at which a liquid transforms into vapour under existing pressure conditions.Column Temperature.Polarity.Carrier Gas Flow Rate.Column Length.Column Diameter.Film Thickness.