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Running a Column Dissolve the sample in the minimum amount of solvent (5?10 drops). ... Using a pipette or syringe with a thick needle, drip the sample directly onto the top of the silica. ... Once the entire sample has been added, allow the column to drain so that the solvent level touches the top of the silica.

HPLC Column equilibration Before running your samples, it is essential to equilibrate the column with the mobile phase. Equilibration involves running the mobile phase through the column for a set period of time to ensure that the stationary phase is fully saturated with the mobile phase.

PROCEDURE Attach the column to the HPLC system with the outlet line flushing to a waste container. Flush the column for 10 column volumes with 50/50 acetonitrile or methanol/water. Connect the column to the detector. Flush the column with the mobile phase for 20 column volumes.

The truth is that column chromatography should take no more than 15 min, including fraction collection and evaporation for scales ranging from 1 mg to 10 g. For particularly difficult separations for 4 or more products with close Rf's for 2 or more compounds in the mixture, add 10 min to your total column time.

Silica gel can be used in column chromatography for the separation of analytes from interfering compounds of a different chemical polarity. It may be used activated, after heating to 150 - 160EC, or deactivated with up to 10% water.

How to wetpack a Column - YouTube YouTube Start of suggested clip End of suggested clip Once you're ready to pack your column you're going to take your pump adapter. And place it on top.MoreOnce you're ready to pack your column you're going to take your pump adapter. And place it on top. And it make sure you use the Keck clip to secure. It. Open up your bleeder.

Running a flash column Choosing the solvent system by TLC analysis. Choosing the right stationary phase. Determining the amount of stationary phase. Choosing the right column and test tubes. Pack the column. Prepare and load your sample. Running the column. Flushing and drying the column.