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How to Clean an HPLC Column in Daily & Deeper Cleaning - YouTube YouTube Start of suggested clip End of suggested clip Around. And you can flow into a container. So you don't dirty your detector. The method for reverseMoreAround. And you can flow into a container. So you don't dirty your detector. The method for reverse flush would be to start with 95. Percent water. And 5 methanol for about 10 column. Volumes.

A much better approach to washing the column is to first replace the buffer component of the mobile phase with water. For example, replace acetonitrile?buffer with acetonitrile?water. Wash the column with 5?10 mL of this ? just enough to wash most of the buffer out.

Using isopropanol as an intermediate solvent is recommended, followed by mobile phase without buffer, then finally with the starting mobile-phase composition. Tetrahydrofuran is another popular solvent that can be used for cleaning contaminated columns.

Regeneration Procedure for Normal-Phase Silica Column Set the flow at 1 mL/min. Flush column with 50 mL of methanol:chloroform. Flush column with 50 mL of ethyl acetate. Reconnect the column in the proper flow direction.

Wash the column with the current mobile phase without buffer, salt or acid for 10-15 min. If the ratio of organic solvent and water is changed, salt may precipitate. Wash impurities in a column to achieve a stable base line: Use a solvent with strong elution properties in which the sample dissolves well.

Typical Regeneration Procedure for Reversed Phase Columns: Disconnect column from detector and run wash solvents into a beaker. Start with your mobile phase without buffer salts (water/organic). ... Next, use 100% organic (methanol or acetonitrile). Check pressure to see if it has returned to normal; if not, then:

How to Clean an HPLC Column in Daily & Deeper Cleaning - YouTube YouTube Start of suggested clip End of suggested clip Lastly you flip the column. Around. And you can flow into a container. So you don't dirty yourMoreLastly you flip the column. Around. And you can flow into a container. So you don't dirty your detector. The method for reverse flush would be to start with 95.