Collection and preservation of human blood Forms for Wayne
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FAQ
Three popular methods of blood collection are: Arterial Sampling. Venipuncture Sampling. Fingerstick Sampling.
Whole blood samples should not remain at room temperature longer than 8 hours. If assays are not completed within 8 hours, samples should be stored at +2°C to +8A°C no longer than 7 days. If assays are not completed within 7 days, or the sample is to be stored beyond 7 days, samples should be frozen at -15A°C to -20A°C.
At a minimum, the equipment required when drawing blood includes gloves, alcohol or iodine to cleanse the area, a tourniquet, tubes, a tube holder, needles, tape, and gauze. A Phlebotomist should be completely aware of the wide range of needles, tubes, etc.
When a blood draw is taken from a vein, it's known as venipuncture. During venipuncture, a lab professional, known as a phlebotomist, will take a blood sample from a vein in your arm, using a small needle. After the needle is inserted, a small amount of blood will be collected into a test tube or vial.
Blood collection for serum by venipuncture and handlingWhole blood can be stored at 48°C for up to 24 hours before the serum is separated, but it must not be frozen. Whole blood should be allowed to clot and then centrifuged at 1000 A— gravitational units (g) for 10 minutes to separate the serum.
Ficoll-Hypaque is a solution of sodium diatrizoate (a dense, iodine-containing molecule) and Ficoll (a polysaccharide) with a density (1.083 g/cc) that falls between that of neutrophils and that of the mononuclear cells.